Mitochondrial Sirt3 Expression is Decreased in APP/PS1 Double Transgenic Mouse Model of Alzheimer's Disease

作者：Yang Wenxiu^*; Zou Yan; Zhang Man; Zhao Nan; Tian Qi; Gu Min; Liu Wei; Shi Rui; Lu Yang; Yu Weihua

来源：Neurochemical Research, 2015, 40(8): 1576-1582.

DOI：10.1007/s11064-015-1630-1

• Sirt3
• Alzheimer's disease
• APP/PS1 double transgenic mouse
• Mitochondrial dysfunction

Emerging data suggests that mitochondrial dysfunction is prominently involved in Alzheimer disease (AD) progression. Sirtuin-3 (Sirt3) is a member of the sirtuin family of nicotinamide adenine dinucleotide dependent deacetylases that regulates a variety of mitochondrial functions and suppresses mitochondria-related physiology. Here, we determined sirt3 expression in a mouse model of AD. Spatial learning and memory were tested by Morris water maze in APP/PS1 double transgenic mice. The expression of sirt3 was assayed by real-time quantitative PCR and western blotting. Age-and gender-matched wild-type (WT) littermates were used as controls. Cortical sirt3 localization was assessed using immunohistochemistry. The expression of sirt3 mRNA was significantly lower in the cortex of APP/PS1 double transgenic mice than in WT littermates (0.83 +/- A 0.24 vs. 1.10 +/- A 0.21, P < 0.05). A comparable reduction was found in sirt3 protein levels using western blotting. The ratio of mean optical density (MOD) of total sirt3/beta-actin in the cortex was 0.77 +/- A 0.11 in APP/PS1 double transgenic mice and 1.34 +/- A 0.17 in the WT littermates (P < 0.01). Immunohistochemistry showed the same change as western blotting. The ratio of MOD of integral optical density/total area in APP/PS1 and WT littermates was 0.58 +/- A 0.02 and 0.71 +/- A 0.05 (P < 0.01). These data show that sirt3 was depleted in APP/PS1 double transgenic mice. The results suggest that mitochondrial sirt3 might participate in the development of AD via mitochondrial dysfunction.

• 出版日期2015-8
• 单位重庆医科大学