A simple multiplex PCR for assessing prevalence of extended-spectrum beta-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran

作者：Ghasemi Younes^*; Archin Talieh; Kargar Mohammad; Mohkam Milad

来源：Asian Pacific Journal of Tropical Medicine, 2013, 6(9): 703-708.

DOI：10.1016/S1995-7645(13)60122-4

• Klebsiella pneumoniae
• ESBLs
• ICUs
• Multiplex PCR

Objective: To identify three common genes (bla(TEM), bla(SHV) and bla(CTX-M)) responsible for ESBL production in Klebsiella pneumoniae (K. pneumoniae)isolated from Intensive Care Units of Namazi Hospital, Shiraz, Iran. Methods: A total of 60 non-repetitive nosocomial isolates from 60 patients were selected during 2009-2010. The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test (DDST) according to CLSI guidelines. The ESBL%26apos;s genotype was then analyzed by multiplex PCR of bla(TEM), bla(SHV) and bla(CTX-M) genes and DNA sequencing. Results: The primary susceptibility tests of K. pneumoniae showed that among 10 examined antibiotics, the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem, respectively. The phenotypic determination of ESBL by DDST showed that 60% (n=36) of isolates produced ESBL. Multiplex PCR of genes among K. pneumoniae isolates showed that 39% (n=18) of them have TEM, 39% (n=18) of them have both CTX-M and TEM and 13% (n=8) of them have TEM, SHV, CTX-M. Conclusions: Our findings reveal the high prevalence (60%) of ESBL producing K. pneumoniae from ICU patients along with a new pattern of bla(TEM) distribution differ from other countries.

• 出版日期2013-9