A sensitive electrochemiluminescent aptasensor based on perylene derivatives as a novel co-reaction accelerator for signal amplification

作者：Yu, Yan-Qing; Zhang, Hai-Yu; Chai, Ya-Qin; Yuan, Ruo^*; Zhuo, Ying^*

来源：Biosensors and Bioelectronics, 2016, 85: 8-15.

DOI：10.1016/j.bios.2016.04.088

• Perylene derivatives
• Co-reaction accelerator
• Graphene-CdTe
• Electrochemiluminescence
• Thrombin

Herein, a novel signal amplification strategy was designed using the perylene derivative as the co-reaction accelerator toward graphene-CdTe quantum dots (G-CdTe)/S2O82- system to construct a highly sensitive electrochemiluminescent (ECL) aptasensor for thrombin (TB) detection. Firstly, the G-CdTe nanocomposites were prepared by one-step method of in situ generating CdTe quantum dots onto the surface of the graphene oxide by using 3-mercaptopropionic acid as the CdTe QDs stabilizer. Then, a kind of perylene derivative (PTC-Lys), was synthesized by covalently binding L-lysine to 3,4,9,10-perylenetetracarboxylic acid, which was further immobilized onto the G-CdTe by the pi-pi* stacking and cross-linked the detection thrombin aptamer (TBA II) to obtain the TBA II/PTC-Lys/G-CdTe signal probes. It is worth pointing out that PTC-Lys acting as an efficient co-reaction accelerator interacted with the co-reactant of S2O82- rather than G-CdTe to promote the more oxidant mediators of SO4 center dot-, which could further react with G-CdTe to produce excited state species G-CdTe* for emitting light. Compared with the G-CdTe/ S2O82- ECL system, our proposed strategy with the introduction of co-reaction accelerator of PTC-Lys exhibited ultra-high sensitivity to quantify the concentration of TB from 1.0 x 10(-7) nM to 10 nM with a detection limit of 34 aM.

• 出版日期2016-11-15
• 单位西南大学