A Comprehensive Whole-Genome Integrated Cytogenetic Map for the Alpaca (Lama pacos)

作者：Avila Felipe; Baily Malorie P; Perelman Polina; Das Pranab J; Pontius Joan; Chowdhary Renuka; Owens Elaine; Johnson Warren E; Merriwether David A; Raudsepp Terje^*

来源：Cytogenetic and Genome Research, 2014, 144(3): 196-207.

DOI：10.1159/000370329

• Alpaca
• Chromosomes
• Comparative
• Cytogenetic
• Dromedary camel
• FISH
• Genome
• Radiation hybrid
• Sequence

Genome analysis of the alpaca (Lama pacos, LPA) has progressed slowly compared to other domestic species. Here, we report the development of the first comprehensive whole-genome integrated cytogenetic map for the alpaca using fluorescence in situ hybridization (FISH) and CHORI-246 BAC library clones. The map is comprised of 230 linearly ordered markers distributed among all 36 alpaca autosomes and the sex chromosomes. For the first time, markers were assigned to LPA14, 21, 22, 28, and 36. Additionally, 86 genes from 15 alpaca chromosomes were mapped in the dromedary camel (Camelus dromedarius, CDR), demonstrating exceptional synteny and linkage conservation between the 2 camelid genomes. Cytogenetic mapping of 191 protein-coding genes improved and refined the known Zoo-FISH homologies between camelids and humans: we discovered new homologous synteny blocks (HSBs) corresponding to HSA1-LPA/CDR11, HSA4-LPA/CDR31 and HSA7-LPA/CDR36, and revised the location of breakpoints for others. Overall, gene mapping was in good agreement with the Zoo-FISH and revealed remarkable evolutionary conservation of gene order within many human-camelid HSBs. Most importantly, 91 FISH-mapped markers effectively integrated the alpaca whole-genome sequence and the radiation hybrid maps with physical chromosomes, thus facilitating the improvement of the sequence assembly and the discovery of genes of biological importance.

• 出版日期2014