Microfluidic Image Cytometry (mu FIC) Assessments of Silver Nanoparticle Cytotoxicity

作者：Park Jonghoon; Yoon Tae Hyun^*

来源：BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2012, 33(12): 4023-4027.

DOI：10.5012/bkcs.2012.33.12.4023

• Microfluidic image cytometry
• Microfluidic device
• Cytotoxicity assessment
• Ag nanoparticles
• Nanotoxicity

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (mu FIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the mu FIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This mu FIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.

• 出版日期2012-12-20