Antioxidant Activity of Uruguayan Propolis. In Vitro and Cellular Assays

作者：Silva Veronica; Genta Gonzalo; Moeller Matias N; Masner Martin; Thomson Leonor; Romero Natalia; Radi Rafael; Fernandes Denise C; Laurindo Francisco R M; Heinzen Horacio; Fierro Walter; Denicola Ana^*

来源：Journal of Agricultural and Food Chemistry, 2011, 59(12): 6430-6437.

DOI：10.1021/jf201032y

• propolis
• antioxidant activity
• ORAC
• polyphenols
• flavonoids
• nitric oxide
• nitric oxide synthase
• NADPH oxidase

The antioxidant capacity of propolis from the southern region of Uruguay was evaluated using in vitro as well as cellular assays. Free radical scavenging capacity was assessed by ORAC, obtaining values significantly higher than those of other natural products (8000 mu mol Trolox equiv/g propolis). ORAC values correlated well with total polyphenol content (determined by Folin-Ciocalteu method) and UV absorption. Total polyphenol content (150 mg gallic acid equiv/g propolis) and flavonoids (45 mg quercetin equiv/g propolis) were similar to values reported for southern Brazilian (group 3) and Argentinean propolis. Flavonoid composition determined by RP-HPLC indicates a strong poplar-tree origin. Samples high in polyphenols efficiently inhibit low-density lipoprotein lipoperoxidation and tyrosine nitration. In addition, Uruguayan propolis was found to induce the expression of endothelial nitric oxide synthase and inhibit endothelial NADPH oxidase, suggesting a potential cardiovascular benefit by increasing nitric oxide bioavailability in the endothelium.

• 出版日期2011-6-22