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摘要
Four novel Gram-stain-negative, rod-shaped, and non-motile bacterial strains, SLG210-21(T), SLG210-4, SLG210-5 and SLG210-14, were isolated from oil-contaminated saline soil in Shengli Oilfield, China. Growth were observed at 25-42 A degrees C (optimum 37 A degrees C), in the presence of 0-10 % (w/v) NaCl (optimum 0-1 %) and at pH 4.0-10.0 (optimum pH 7.6-8.6). All the strains were positive for catalase and alpha, beta-galactosidase activities and nitrogen reduction, and negative for oxidase activity, glucose fermentation and hydrolysis of agar, starch, gelatin, Tween 40, 60 and 80. The DNA G+C contents of the four strains were 41.3-43.0 mol% and the predominant respiratory quinones were all menaquinone-7. The major fatty acids were iso-C-15:0, anteiso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:1 omega 5c and summed feature 9 (iso-C-17:1 omega 9c and/or 10-methyl C-16:0), while the polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, glycolipid, two unidentified phospholipids and two unidentified amino lipids. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains clustered together to form a stable branch in the family Cyclobacteriaceae, and were most closely related to the genera Cyclobacterium and Echinicola with the 16S rRNA gene sequence similarities being 88.6-90.3 and 89.6-91.4 %, respectively. DNA-DNA hybridization between SLG210-21(T) and the other three strains showed the relatedness of 93.8 +/- A 4.5, 96.2 +/- A 4.2 and 82.3 +/- A 4.8 %, respectively. Based on the polyphasic analysis, a novel species in a new genus, Negadavirga shengliensis gen. nov., sp. nov., is proposed with SLG210-21(T) (=LMG 27734(T) = CGMCC1.12768(T)) as the type strain.
- 出版日期2015-3
- 单位中国科学院微生物研究所; 北京大学
