科研之友
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摘要
PC12 cells were used to examine the in vitro antioxidative and anti-inflammatory effects of oleanolic acid (OA) and ursolic acid (UA). PC12 cells were pretreated with OA or UA at 20 and 40 mu M and followed by exposure of hydrogen peroxide (H2O2) or 1-methyl-4-phenylpyridinium ion (MPP+) to induce cell injury. Results showed that H2O2- or MPP+-treatment significantly decreased cell viability and increased lactate dehydrogenase (LDH) release (P < 0.05). The pretreatment from OA or UA significantly and concentration-dependently reduced subsequent H2O2- or MPP+-induced cell death and LDH release (P < 0.05). Either H2O2- or MPP+-treatment significantly increased malonyldialdehyde (MDA) formation, decreased glutathione (GSH) content, and diminished glutathione peroxidase (GPX), catalase, and superoxide dismutase (SOD) activities (P < 0.05). The pretreatment from OA or UA significantly retained GSH, and reversed H2O2- and MPP+-induced impairment in catalase and SOD activities (P < 0.05), and decreased MDA formation (P < 0.05). Either H2O2- or MPP+-treatment significantly elevated interleukin-6 (IL-6) and tumor necrosis factor (TNF)-alpha levels (P < 0.05). The pretreatments from OA or UA significantly attenuated subsequent H2O2- or MPP+-induced release of IL-6 and TNF-alpha (P < 0.05). Based on the observed antioxidative and anti-inflammatory activities from OA and UA, these 2 compounds were potent agents against neu rodegenerative disorder.
