Direct electrochemistry of cytochrome c on nanotextured diamond surface

作者：Yang Nianjun^*; Hoffmann Rene; Smirnov Waldemar; Kriele Armin; Nebel Christoph E

来源：Electrochemistry Communications, 2010, 12(9): 1218-1221.

DOI：10.1016/j.elecom.2010.06.023

• Cytochrome c
• Peroxidase activity
• Nano-structures
• Diamond electrochemistry

Nanotextured diamond surfaces with geometrical properties close to protein dimensions were used for the realization of direct electron transfer of cytochrome c (cyt c) without any covalent bonding. The peroxidase activity of native and denatured cyt c was also investigated. Cyclic voltammograms of native cyt c show quasi-reversible electron transfer reactions, while no heme redox activity is detected for denatured cyt c. Unfolding (denaturation) of cyt c can be achieved in the presence of hydrogen peroxide. Partially or fully denatured cyt c showed higher peroxidase activity than native cyt c. This is because denatured cyt c loses its tertiary structure and hydrogen peroxide is easier to access the heme redox center. The apparent Michaelis-Menten constant K(m) for native and denatured cyt c has been determined to be 0.23 mM and 0.08 mM.

• 出版日期2010-9