Proteomics strategy for quantitative protein interaction profiling in cell extracts

作者：Sharma Kirti; Weber Christoph; Bairlein Michaela; Greff Zoltan; Keri Gyoergy; Cox Juergen; Olsen Jesper V; Daub Henrik^*

来源：Nature Methods, 2009, 6(10): 741-U13.

DOI：10.1038/NMETH.1373

We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an antibody as affinity ligands.

• 出版日期2009-10