Analysis of Primitive Erythroid Cell Proliferation and Enucleation Using a Cyan Fluorescent Reporter in Transgenic Mice

作者：Vacaru Andrei M; Isern Joan; Fraser Stuart T; Baron Margaret H^*

来源：Genesis, 2013, 51(11): 751-762.

DOI：10.1002/dvg.22420

• primitive erythropoiesis
• transgenic mice
• cyan fluorescent protein
• histone fusion
• fluorescent reporter
• imaging

Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this epsilon-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The epsilon-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The epsilon-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage. genesis 51:751-762.

• 出版日期2013-11