Analytical ultracentrifugation studies of oligomerization and DNA-binding of TtCarH, a Thermus thermophilus coenzyme B-12-based photosensory regulator

作者：Diez Ana I; Manuel Ortiz Guerrero Juan; Ortega Alvaro; Elias Arnanz Montserrat; Padmanabhan S; Garcia de la Torre Jose

来源：European Biophysics Journal, 2013, 42(6): 463-476.

DOI：10.1007/s00249-013-0897-x

• Analytical ultracentrifugation
• Hydrodynamics
• Light-dependent self-association
• CarH
• Thermus thermophilus

Thermus thermophilus transcriptional factor TtCarH belongs to a newly discovered class of photoreceptors that use 5%26apos;-deoxyadenosylcobalamin (AdoB(12)) as the light-sensing chromophore. Photoregulation relies on the repressor activity of AdoB(12)-bound oligomers in the dark, which light counteracts by oligomer disruption due to AdoB(12) photolysis. In this study, we investigated TtCarH self-association and binding to DNA in the dark and in the light using analytical ultracentrifugation (AUC) methods, both sedimentation velocity (SV) as well as equilibrium (SE). From a methodological point of view, this study shows that AUC can provide hydrodynamic insights in cases where light is a crucial determinant of solution properties. For the light-sensitive TtCarH, absorbance as well as interference AUC data yielded comparable results. Sedimentation coefficients and whole-body hydrodynamic analysis from SV experiments indicate that in solution apo-TtCarH and light-exposed AdoB(12)-TtCarH are predominantly aspherical, ellipsoidal monomers, in accord with SE data. By comparison, AdoB(12)-TtCarH exists as a more compact tetramer in the dark, with smaller forms such as dimers or monomers remaining undetected and low levels of larger oligomers appearing at higher protein concentrations. AUC analyses indicate that in the dark AdoB(12)-TtCarH associates as a tetramer with DNA but forms smaller complexes in the apo form or if exposed to light. The self-association and DNA-binding properties of TtCarH deduced from AUC are consistent with data from size-exclusion and DNA-binding gel-shift assays. AUC analyses together with hydrodynamic modeling provide insights into the AdoB(12)- and light-dependent self-association and DNA-binding of TtCarH.

• 出版日期2013-6