Analysis of tea catechins in vegetable oils by high-performance liquid chromatography combined with liquid-liquid extraction

作者：Zhang, Xin^*; Wu, Zufang; Weng, Peifang; Yang, Yang

来源：International Journal of Food Science and Technology, 2015, 50(4): 885-891.

DOI：10.1111/ijfs.12726

• high-performance liquid chromatography
• liquid-liquid extraction
• tea catechins
• vegetable oil

In this study, a method was developed for the determination of various tea catechins in vegetable oils. Firstly, vegetable oils including tea seed oil, sunflower seed oil and soya bean oil were extracted by methanol/water (40:60, v/v), and then, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of GA, caffeine, EGC, EGCG, EC, ECG, GC, GCG, C and CG. For the compounds detected in tested vegetable oils, LODs were in the range of 0.05-1.65ng, both intraday and interday relative standard deviations (RSDs) were <5.0%, and the recovery rates were in the range of 96.2-100.5% with RSD <3.7%. The results showed in vegetable oils which declared to had added tea catechins in, the concentrations of tea catechins were less than that showed in package label, and the content of EGCG was the highest in all samples. Therefore, the advancement made in our study will facilitate studies of tea catechins in oil industry.

• 出版日期2015-4
• 单位宁波大学