Hydrogen sulfide improves left ventricular function in smoking rats via regulation of apoptosis and autophagy

作者：Zhou Xiang^*; An Guoyin; Chen Jianchang

来源：Apoptosis, 2014, 19(6): 998-1005.

DOI：10.1007/s10495-014-0978-z

• Hydrogen sulfide
• Left ventricular function
• Smoking
• Apoptosis
• Autophagy

The present study was designed to investigate the protective effects of hydrogen sulfide (H2S) against cigarette smoking-induced left ventricular dysfunction in rats. Left ventricular structure and function were assessed using two-dimensional echocardiography. Cardiomyocyte apoptosis was determined by Annexin V/PI and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining. Cardiac autophagy was evaluated by detection of autophagy-related protein expression and observation of autophagosomes. Our results indicated that administration of NaHS (a donor of H2S) could protect against smoking-induced left ventricular systolic dysfunction. H2S was found to exert anti-apoptotic effects in the myocardium of smoking rats by inhibiting JNK and P38 mitogen-activated protein kinases pathways and activating PI3K/Akt signaling. Moreover, H2S could also reduce smoking-induced autophagic cell death via regulation of AMPK/mTOR signaling pathway. In conclusion, our study demonstrates that H2S can improve left ventricular systolic function in smoking rats via regulation of apoptosis and autophagy.

• 出版日期2014-6
• 单位苏州大学