An Apolipophorin III Protein from the Hemolymph of Desert Locust, Schistocerca gregaria

作者：Malik Zulfiqar A^*; Amir Sumaira

来源：Applied Biochemistry and Biotechnology, 2011, 165(7-8): 1779-1788.

DOI：10.1007/s12010-011-9394-9

• Apolipophorin III
• Locusta migratoria
• Schistocerca gregaria
• Hemolymph

Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at similar to 19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.

• 出版日期2011-12