Analysis of Rocuronium in Human Whole Blood and Tissues Using Liquid Chromatography-Tandem Mass Spectrometry

作者：Cho Hwang Eui; Park Mee Jung; Kim Sun Cheun; Hong Ran Seon; Moon Dong Cheul; Ahn Su Youn^*

来源：Journal of Chromatographic Science, 2013, 51(4): 297-301.

DOI：10.1093/chromsci/bms252

A rapid, accurate and sensitive liquid chromatography-tandem mass spectrometry method has been developed for the determination of a quaternary nitrogen muscle relaxant, rocuronium, in human blood. The procedure involves protein precipitation with chloroform and trichloroacetic acid, and purification using methanol. The chromatography was performed using a phenyl-hexyl column (150 3 2.0 mm i.d., 3 mu m; Phenomenex) with a mobile phase consisting of 5 mM ammonium formate (pH 3.0) and acetonitrile. Multiple reaction monitoring was used for quantification. The assay was linear over a concentration range of 4-500 ng/mL for rocuronium with R-2 %26gt;= 0.998. The recoveries for this compound ranged from 96.0 to 109.1%. The intra-day and inter-day precision was less than 10.5% and the accuracy ranged from 106.6 to 114.9%. The validated method was applied to quantify the content of rocuronium in blood and a variety of tissues of a victim suspected of overdose. In conclusion, the method was successfully applied for the analysis of rocuronium in biological samples for forensic toxicology.

• 出版日期2013-4