Novel microcalorimetric assay for antibacterial activity of implant coatings: The cases of silver-doped hydroxyapatite and calcium hydroxide

作者：Braissant Olivier^*; Chavanne Philippe; de Wild Michael; Pieles Uwe; Stevanovic Sabrina; Schumacher Ralf; Straumann Lukas; Wirz Dieter; Gruner Philipp; Bachmann Alexander; Bonkat Gernot

来源：Journal of Biomedical Materials Research Part B: Applied Biomaterials , 2015, 103(6): 1161-1167.

DOI：10.1002/jbm.b.33294

• antibacterial implant coatings
• hydroxyapatite
• calcium hydroxide
• isothermal microcalorimetry
• Staphylococcus

Biomaterials with antimicrobial properties are now commonly used in different clinical specialties including orthopedics, endodontic, and traumatology. As a result, assessing the antimicrobial effect of coatings applied on implants is of critical importance. In this study, we demonstrate that isothermal microcalorimetry (IMC) can be used for monitoring bacterial growth and biofilm formation at the surface of such coatings and for determining their antimicrobial effects. The antibacterial effects of silver doped hydroxyapatite (HA) and calcium hydroxide coatings on Staphylococcus epidermidis were determined with a minimal workload. Using the Gompertz growth model we determined biofilm growth rates close to those values reported in the literature. Furthermore, we were able to estimate the reduction in the bacterial inocula originally applied at the surface of the coatings. Therefore, in addition to monitoring the antimicrobial effect of silver doped HA and calcium hydroxide coatings, we also demonstrate that IMC might be a valuable tool for assessing such antimicrobial properties of implant coatings at a minimal workload.

• 出版日期2015-8