Detecting quinoxaline-2-carboxylic acid in animal tissues by using sensitive rapid enzyme-linked immunosorbent assay and time-resolved fluoroimmunoassay

作者：Le, Tao^*; Yu, Huan; Niu, Xiaodong

来源：Food Chemistry, 2015, 175: 85-91.

DOI：10.1016/j.foodchem.2014.11.135

• Carbadox
• Quinoxaline-2-carboxylic acid
• Indirect competitive enzyme-linked immunosorbent assay
• Time-resolved fluoroimmunoassay
• Monoclonal antibody
• Animal tissue

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluoroimmunoassay (TR-FIA) based on an anti-N-butylquinoxaline-2-carboxamide (BQCA) monoclonal antibody were standardized and validated for quinoxaline-2-carboxylic acid (QCA) screening in animal tissues and its performance were compared to HPLC. The sensitivities obtained for edible tissue extracts were 1.62 and 1.12 ng ml(-1) for ic-ELISA and TR-FIA detection, respectively. Two samples were spiked with QCA and analyzed by both methods. The recovery values ranged from 92.6% to 112.2% and the coefficients of variation were less than 15% for QCA spiking into swine tissue samples at concentrations of 2.5-50.0 mu g kg(-1). Excellent correlations (r(2) = 0.987-0.996) of the ic-ELISA/HPLC and TR-FIA/HPLC data were observed for processed samples. The results demonstrated that the ic-ELISA and TR-FIA methods were rapid and accurate for the residue detection of QCA in animal tissues.

• 出版日期2015-5-15
• 单位重庆师范大学