Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells

作者：Zhao, Xiaohong^*; Yang, Li; Hu, Jianguo

来源：Journal of Experimental & Clinical Cancer Research, 2011, 30: 55.

DOI：10.1186/1756-9966-30-55

• miR-27a drug resistance
• porliferation
• gastric carcinoma

Aims: Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells.
Methods: The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc.
Results: Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Downregulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21.
Conclusions: MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy.

• 出版日期2011-5-13
• 单位宁夏医科大学