NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulates paracellular transport

作者：Tokuda Shinsaku^*; Miyazaki Hiroaki; Nakajima Ken ichi; Yamada Toshiki; Marunaka Yoshinori

来源：Biochemical and Biophysical Research Communications, 2010, 393(3): 390-396.

DOI：10.1016/j.bbrc.2010.02.002

• Tight junction
• Claudin
• Paracellular transport
• Occludin
• NaCl

In multicellular organisms, epithelia separate and divide the internal environment maintaining appropriate conditions in each compartment. To maintain homeostasis in these compartments, claudins, major cell adhesion molecules in tight junctions (TJs), regulate movements of several substances through the paracellular pathway (barrier function). In this study, we investigated effects of the flux of several substances between apical and basolateral side on paracellular transport and TJ protein localization. NaCl flux from apical to basolateral side increased paracellular conductance (Gp) and recruited claudin-1 from lateral cell membrane to the apical end with the colocalization with occludin, one of the TJ proteins concentrated at TJ strands. Oppositely-directed flux of sucrose against NaCl flux inhibited these reactions and same directional flux of sucrose with NaCl enhanced the increase of Gp, whereas 10-kDa dextran inhibited these reactions regardless of the side of administration. Our present findings indicated that TJ protein localization and barrier function are regulated depending on the environmental differences between apical and basolateral side.

• 出版日期2010-3-12