科研之友
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摘要
Foliar chlorosis caused by iron deficiency of plants grown on calcareous soil results in substantial crop yield loss and is an important in crop production problem. The objective of this study was to identify the quantitative trait locus (QTL) controlling resistance to iron deficiency chlorosis (IDC) in mungbean. An RIL population of 122 F-8 lines developed from the cross between a susceptible cultivar, "Kamphaeng Saen 2" and a resistant line,"NM10-12" was genetically analyzed with simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The population was evaluated for IDC resistance in an iron deficient field by visually scoring and SPAD measurements in 2010 and 2011. Segregation of the visual scores and SPAD values of the RILs in both years suggested quantitative inheritance of the resistance to IDC. Visual score and SPAD value from each year and combined data were used for QTL analysis. Single marker analysis revealed that 12 DNA markers from 3 linkage groups (LG) associated with the resistance. Four SSR and two AFLP markers on LG 3 associated with the resistance in all cases. Composite interval mapping identified two QTLs, qIDC3.1 and qIDC2.1, controlling the resistance. qIDC3.1 on LG 3 was identified from visual scores and SPAD values to account for 12.12% and 41.67% of the total variation depending on traits measured and years. qIDC2.1 on LG 2 was detected only from visual score data in 2010 and explained 45.66% of the total variation. The qIDC3.1 was the same as gIR which was the major QTL previously reported for IDC in mungbean grown in hydroponic conditions. The SSR markers CEDG084 and CEDC031 flanked and closely linked to the qIDC are useful for marker-assisted selection for mungbean resistance to IDC.
- 出版日期2012-10-20
