A report of a galactan from marine alga Gelidium crinale with in vivo anti-inflammatory and antinociceptive effects

作者：de Sousa Albertina A S; Benevides Norma M B; Pires Alana de Freitas; Fiuza Felipe P; Queiroz Maria G R; Morais Thamires M F; Pereira Maria G; Assreuy Ana M S^*

来源：Fundamental & Clinical Pharmacology, 2013, 27(2): 173-180.

DOI：10.1111/j.1472-8206.2011.01001.x

• Gelidium crinale
• inflammation
• pain
• sulfated galactan

The sulfated galactan of the red marine alga Gelidium crinale (SG-Gc) was purified by ion exchange chromatography and tested by intravenous (i.v.) route in rodent experimental models of inflammation and nociception. The anti-inflammatory activity of SG-Gc (0.01, 0.1 and 1mg/kg) was evaluated in the model of rat paw edema induced by different inflammatory stimuli, while SG-Gc (0.1, 1 and 10mg/kg) antinociceptive effect was assessed in models of nociception/hyperalgesia elicited by chemical (formalin test), thermal (hot plate), and mechanical (von Frey) stimuli in mice. In addition, the toxicity was evaluated after rat treatment with SG-Gc (1mg/kg; i.v.) during 10days, followed by analysis of the wet weight of animal's body/organs and hematological/biochemical parameters. Sulfated galactan of G.crinale inhibited the time course of dextran-induced paw edema, at all doses, showing maximal effect at 1mg/kg (42%) and that induced by carrageenan at 0.01 (18%) and 1mg/kg (20%), but was ineffective on the edema elicited by zymosan. At the highest dose, SG-Gc also inhibited the paw edema induced by histamine (49%), compound 48/80 (32%), and phospholipase A2 (44%). Sulfated galactan of G.crinale inhibited both neurogenic and inflammatory phases of the formalin test, at all doses, and at 10mg/kg, the animals flinch reaction in the von Frey test in the 1st and 3rdh by 19 and 26%, respectively. Additionally, SG-Gc treatment was well tolerated by animals. In conclusion, SG-Gc presents anti-inflammatory effect involving the inhibition of histamine and arachidonic acid metabolites and also antinociceptive activity, especially the inflammatory pain with participation of the opioid system.

• 出版日期2013-4