Facile immobilization of nitrile hydratase in SBA-15 via a biomimetic coating

作者：Chen, Yan; Jiang, Yanjun; Gao, Junkai^*; Wu, Wenfeng; Dong, Liangxiong; Yang, Zejun

来源：Journal of Porous Materials, 2017, 24(3): 787-793.

DOI：10.1007/s10934-016-0317-6

• Polyopamine
• Biomimetic coating
• Facile immobilization
• Nitrile hydratase

Dopamine functionalized mesoporous molecular sieve SBA-15 (Dop-SBA-15), synthesized by a simple and cost-effective method, was developed as a matrix to immobilize NHase (NHase@Dop-SBA-15). Various characterization techniques such as SEM, TEM, BET and FT-IR were carried out to investigate the properties of Dop-SBA-15 and NHase@Dop-SBA-15. It was shown that the Dop-SBA-15 had highly ordered mesopores and large surface area of 447 m(2)/g. The activity, thermal stability, pH stability, storage stability, and reusability of NHase@Dop-SBA-15 were studied in detail. It was observed that the performance of NHase@Dop-SBA-15 was improved significantly compared to that of the free NHase and NHase immobilized in unfunctionalized SBA-15. This study provided insight into the preparation of Dop-SBA-15 and its application for NHase immobilization.

• 出版日期2017-6
• 单位河北工业大学; 浙江海洋大学