Analysis of Interleukin-8 Gene Variants Reveals Their Relative Importance as Genetic Susceptibility Factors for Chronic Periodontitis in the Han Population

作者：Zhang, Nan^*; Xu, Yuehong; Zhang, Bo; Zhang, Tianxiao; Yang, Haojie; Zhang, Bao; Feng, Zufei; Zhong, Dexing

来源：PLos One, 2014, 9(8): e104436.

DOI：10.1371/journal.pone.0104436

• RED-COMPLEX PERIODONTOPATHOGENS
• SINGLE-NUCLEOTIDE POLYMORPHISM
• GINGIVAL CREVICULAR FLUID
• SCHIZOPHRENIA SUSCEPTIBILITY
• RISK-FACTORS
• ASSOCIATION
• DISEASE
• HAPLOTYPES
• PROMOTER
• SMOKING

Interleukin (IL)-8, an important chemokine that regulates the inflammatory response, plays an important role in periodontitis. Previous studies indicate that certain IL-8 gene polymorphisms are associated with periodontitis susceptibility in some populations. However, the literature is somewhat contradictory, and not all IL-8 polymorphisms have been examined, particularly in Han Chinese individuals. The aim of this study was to investigate the association of every IL-8 SNP with chronic periodontitis in Han Chinese individuals. We analyzed 23 SNPs with minor allele frequency (MAF)>= 0.01, which were selected from 219 SNPs in the NCBI dbSNP and preliminary HapMap data analyses from a cohort of 400 cases and 750 controls from genetically independent Han Chinese individuals. Single SNP, haplotype and gender-specific associations were performed. We found that rs4073 and rs2227307 were significantly associated with chronic periodontitis. Further haplotype analysis indicated that a haplotype block (rs4073-rs2227307-rs2227306) that spans the promoter and exon1 of IL-8 was highly associated with chronic periodontitis. Additionally, the ATC haplotype in this block was increased 1.5-fold in these cases. However, when analyzing the samples by gender, no significant gender-specific associations in IL-8 were observed, similar to the results of haplotype association analyses in female and male subgroups. Our results provide further evidence that IL-8 is associated with chronic periodontitis in Han Chinese individuals. Furthermore, our results confirm previous reports suggesting the intriguing possibilities that IL-8 plays a role in the pathogenesis of chronic periodontitis and that this gene may be involved in the etiology of this condition.

• 出版日期2014-8-7
• 单位西安交通大学