Nardostachys jatamansi extract protects against cytokine-induced beta-cell damage and streptozotocin-induced diabetes

作者：Song Mi Young; Bae Ui Jin; Lee Bong Hee; Kwon Kang Beom; Seo Eun A; Park Sung Joo; Kim Min Sun; Song Ho Joon; Kwon Keun Sang; Park Jin Woo; Ryu Do Gon; Park Byung Hyun^*

来源：World Journal of Gastroenterology, 2010, 16(26): 3249-3257.

DOI：10.3748/wjg.v16.i26.3249

• Nardostachys jatamansi
• Cytokines
• Streptozotocin
• Pancreatic beta cells
• Nuclear factor kappa B
• Nitric oxide
• Diabetes mellitus

AIM: To investigate the anti-diabetogenic mechanism of Nardostachys jatamansi extract (NJE).
METHODS: Mice were injected with streptozotocin via a tail vein to induce diabetes. Rat insulinoma RINm5F cells and isolated rat islets were treated with interleukin-1 beta and interferon-gamma to induce cytotoxicity.
RESULTS: Treatment of mice with streptozotocin resulted in hyperglycemia and hypoinsulinemia, which was confirmed by immunohistochemical staining of the islets. The diabetogenic effects of streptozotocin were completely abolished when mice were pretreated with NJE. Inhibition of streptozotocin-induced hyperglycemia by NJE was mediated by suppression of nuclear factor (NF)-kappa B activation. In addition, NJE protected against cytokine-mediated cytotoxicity. Incubation of RINm5F cells and islets with NJE resulted in a significant reduction in cytokine-induced NF-kappa B activation and downstream events, inducible nitric oxide synthase expression and nitric oxide production. The protective effect of NJE was further demonstrated by the normal insulin secretion of cytokine-treated islets in response to glucose.
CONCLUSION: NJE provided resistance to pancreatic beta-cell damage from cytokine or streptozotocin treatment. The beta-cell protective effect of NJE is mediated by suppressing NF-kappa B activation.

• 出版日期2010-7-14