A novel assay for influenza virus quantification using surface plasmon resonance

作者：Nilsson C Estmer; Abbas S; Bennemo M; Larsson A; Hamalainen M D; Frostell Karlsson A^*

来源：Vaccine, 2010, 28(3): 759-766.

DOI：10.1016/j.vaccine.2009.10.070

• Influenza vaccine
• Hemagglutinin
• Quantification
• Biosensor

Quantification of hemagglutinin (HA) by single-radial immuno diffusion (SRID) is the predominant method to ensure product potency in seasonal influenza vaccines. Here a new method for quantification of influenza virus using biosensor technology is presented The method employs quantification of virus via an inhibition assay format using HA proteins for H1N1, H3N2 and B immobilized on a sensor chip. Initial results showed the assay to have higher sensitivity (detection range 0.

• 出版日期2010-1-8