Abnormal expression, highly efficient detection and novel truncations of midkine in human tumors, cancers and cell lines

作者：Tao Ping^*; Xu Donghui; Lin Shuibin; Ouyang Gao Liang; Chang Youde; Chen Qin; Yuan Yuanyang; Zhuo Xinming; Luo Qicong; Li Jie; Li Baoan; Ruan Lingjuan; Li Qifu; Li Zhixing

来源：Cancer Letters, 2007, 253(1): 60-67.

DOI：10.1016/j.canlet.2007.01.019

• truncated midkine
• tumorigenesis/carcinogenesis
• tumor makers
• alternative splicing
• nested-touchdown PCR

We detected aberrant Midkine (MK) expressions in human insulinoma and pancreatic cancer tissues by immunohistochemistry, revealing its potential role in tumorigenesis/carcinogenesis. With a nested-touchdown PCR program we were able to detect the tMK in all human tumor/cancer tissues and cancer/tumor cell lines. Detection of MK in the peripheral cells and precancerous lesions implies its potential for early cancer/tumor diagnosis. Furthermore, we have discovered two novel truncations of the MK, tMKB and tMKC, respectively, in the disease specimens. Our data not only provide an efficient methodology potentially for clinical application but also shed light on the molecular mechanism underlying the role for MK in tumorigenesis/carcinogenesis.

• 出版日期2007-8-8
• 单位福建医科大学; 厦门大学