A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation

作者：Patel Ashaben; Gaudana Ripal; Mitra Ashim K^*

来源：Journal of Microencapsulation, 2014, 31(6): 542-550.

DOI：10.3109/02652048.2014.885606

• Antibody fragment
• dextran sulphate
• hydrophobic ion-pairing complex
• IgG-Fab fragment
• nanoparticles
• protein

IgG-Fab fragment, a model antibody protein was hydrophobically modified by a novel approach of ion-pairing complexation. Three different sulphated ion-pairing agents were utilised including sodium dodecyl sulphate, taurocholic acid and dextran sulphate (DS). The formations of hydrophobic ion-pairing (HIP) complexes were dependant on pH and molar ratio of ion-pairing agent to Fab. Aqueous solubilities of HIP complexes were very low compared to Fab alone. In particular, when dextran sulphate was added as ion-pairing agent, formed Fab: DS HIP complexes were least soluble in water. Further, nanoparticles (NPs) loaded with drug and Fab: DS HIP complex were prepared and characterised with respect to encapsulation efficiency and size. We observed significant improvement in encapsulation efficiency for Fab: DS HIP complex-loaded nanoparticles. This study demonstrates a novel approach of formulating antibody-loaded nanoparticles which can also be employed for delivery of large antibodies.

• 出版日期2014