Association between interleukin-13 gene polymorphism and neonatal asthma in Han nationality Chinese population

作者：Wan, Aiying^*; Yao, Baozhen; Zhang, Xiao; Li, Li

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(2): 2208-2214.

• IL-13
• polymorphism
• neonatal asthma

Background and objective: Interleukin-13 (IL-13) is an important cytokine secreted from type 2 helper T lymphocytes. Previous studies showed that polymorphisms in the IL-13 gene were associated with allergic asthma. The relationship between this marker and neonatal asthma has not been evaluated. So the aim of this study is to analyze the association of IL-13 gene polymorphisms (rs20541 and rs1800925) and the risk of neonatal asthma. Methods: This case-control study included 103 neonatal asthma patients and 125 healthy controls that were matched with the patients by age and gender. The genotyping of IL-13 gene polymorphisms was conducted with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The distribution difference of SNP genotypes and alleles between case and control groups was checked by the chi-square test and genotype frequencies difference in control group was assessed by Hardy-Weinberg equilibrium (HWE). Associations between IL-13 gene polymorphisms and neonatal asthma risk were evaluated using Chi-square test and presented by odds ratio and 95% confidence intervals (CIs). Results: The genotype distributions of selected controls in IL-13 polymorphisms conformed to HWE. Both CT genotype and TT genotype of rs1800925 were frequently detected in cases than in controls, and they were associated with the occurrence of neonatal asthma. Rs1800925 T allele may act as a risk factor for neonatal asthma. Beside, both GA genotype and AA genotype of rs20541 were frequently detected in cases than in controls, but they were not associated with the occurrence of neonatal asthma. In contrast, GT and TT genotypes apparently correlated with the risk of neonatal asthma. However, we did not find significant difference between the cases and controls based on allele. At the same time, these two polymorphisms presented the linkage disequilibrium (LD) and the haplotype T-G obviously increased the risk of neonatal asthma. Conclusions: IL-13 gene rs1800925 polymorphism was associated with neonatal asthma susceptibility. T-G haplotype of rs1800925 and rs20541 might influence the occurrence of neonatal asthma.

• 出版日期2016
• 单位武汉大学