A MALDI-MS-based quantitative glycoprofiling method on a 96-well plate platform

作者：Kim Kyoung Jin; Kim Yoon Woo; Park Han Gyu; Hwang Cheol Hwan; Park In Young; Choi Kwon Young; Yang Yung Hun; Kim Young Hwan; Kim Yun Gon

来源：Journal of Industrial and Engineering Chemistry, 2017, 46: 150-156.

DOI：10.1016/j.jiec.2016.10.025

• High-throughput analysis
• MALDI-MS
• N-glycan
• UPLC-FLR
• Biopharmaceuticals

Here, we developed a high-throughput MALDI-MS based quantitative targeted glycomics (HT MALDI-QTaG) method for analyzing total N-glycans. Although the chemical derivatization processes (i.e., neutralization of sialic acid and incorporation of a positively-charged moiety) were performed in a 96-well with built-in 10 kDa MWCO membrane filters, the quantitative linearity was still quite good (R-2 > 0.99) between varying amounts of the target glycoprotein and the MALDI peak intensities. In addition, we validated the relative quantitative reproducibilities in different well positions in a 96-well plate. As a proof-of-concept, the proposed HT MALDI-QTaG method was successfully used to analyze bovine fetuin, human serum and Enbrel (R).

• 出版日期2017-2-25