Molecular cloning, functional characterization and phylogenetic analysis of TRAIL in Japanese pufferfish Takifugu rubripes

作者：Li, J. F.; Ai, H. X.; Zhang, J.; Du, M. X.; Zhang, Z.; Zhang, J. X.; Zhang, S. Q.^*

来源：Journal of Fish Biology, 2011, 79(3): 747-760.

DOI：10.1111/j.1095-8649.2011.03058.x

• 3D structure
• apoptosis
• cDNA
• SUMO

In this study, the complementary DNA (cDNA) of Japanese pufferfish Takifugu rubripes tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) was cloned by reverse-transcription PCR. The open reading frame of the TRAIL consisted of 870 bases. The deduced amino-acid sequence of the TRAIL showed a high homology with the sequences of other teleosts. Recombinant soluble TRAIL was fused with a small ubiquitin-related modifier gene to enhance the soluble expression level in Escherichia coli BL21 (DE3). In vitro, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide (MTT) assay indicated that the purified soluble TRAIL was able to induce apoptosis of Jurkat and HeLa cells in a dose-dependent manner.

• 出版日期2011-9
• 单位南京师范大学