Selective detection and complete identification of triglycerides in cortical bone by high-resolution H-1 MAS NMR spectroscopy

作者：Mroue Kamal H; Xu Jiadi; Zhu Peizhi; Morris Michael D; Ramamoorthy Ayyalusamy^*

来源：Physical Chemistry Chemical Physics, 2016, 18(28): 18687-18691.

DOI：10.1039/c6cp03506j

Using H-1-based magic angle spinning solid-state NMR spectroscopy, we report an atomistic-level characterization of triglycerides in compact cortical bone. By suppressing contributions from immobile molecules present in bone, we show that a H-1-based constant-time uniform-sign cross-peak (CTUC) two-dimensional COSY-type experiment that correlates the chemical shifts of protons can selectively detect a mobile triglyceride layer as the main component of small lipid droplets embedded on the surface of collagen fibrils. High sensitivity and resolution afforded by this NMR approach could be potentially utilized to investigate the origin of triglycerides and their pathological roles associated with bone fractures, diseases, and aging.

• 出版日期2016-7-28