In vitro mechanistic study of endosulfan-induced spermatogenic cell apoptosis in the mouse

作者：Xu, Ying; Wang, Na; Shi, Zhi-Xiong; Li, Yan-Bo; Zhou, Xian-Qing^*; Sun, Zhi-Wei

来源：Toxicology and Industrial Health, 2016, 32(9): 1550-1563.

DOI：10.1177/0748233714567525

• Endosulfan
• spermatogenic cells
• apoptosis
• mechanism

To investigate the mechanisms of endosulfan-induced reproductive toxicity, the spermatogenic cell lines (GC-1 spg) of mice were treated with 0, 6, 12, and 24 mu g/ml endosulfan for 24 h in vitro. The results showed that endosulfan induced apoptosis as well as oxidative stress and mitochondrial dysfunction. Reactive oxygen species and damage of mitochondria! structure were considered as major factors to GC-1 spg cells apoptosis. We further examined the expression of apoptosis-related proteins in mitochondria pathway by Western blot and immunohistochemistry analysis as well as activities. The results showed that endosulfan significantly improved the expressions of cytochrome c and B-cell lymphoma 2 (Bcl-2)-associated X protein and increased the activities of caspases 9 and 3 as well as the downregulation of the expression of Bcl-2 in GC-1 spg cells. The results suggested that exposure to endosulfan might induce the apoptosis of spermatogenic cells via mitochondria dependent pathway mediated by oxidative stress resulting in the damage of mitochondrial structure and mitochondrial dysfunction.

• 出版日期2016-9
• 单位首都医科大学