科研之友
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摘要
Campylobacter jejuni and Campylobacter coli strains, isolated from slaughtered chickens and human feces, were examined for their susceptibility to six antibiotics (ampicillin, cefotaxime, erythromycin, levofloxacin, nalidixic acid and trimethoprim) by broth microdilution, for minimum inhibitory concentration determination, and disc diffusion assays. For the 32 C. jejuni isolates, the highest levels of resistance were to trimethoprim (65.6% of the isolates by broth microdilution and 62.5% by disc diffusion) and cefotaxime (62.5% by both methods). Comparable levels of resistance to these antibiotics were found in the 24 C. coli isolates. Statistically significant differences were found between all C. coli isolates for cefotaxime (P = 0.0043) using disc diffusion. A high proportion of C. jejuni isolates show resistance to nalidixic acid using both the broth microdilution (59.4%) and disc diffusion (56.2%) methods. C. coli strains were resistant to this antibiotic showing proportions of 75% when tested by broth microdilution and 70.8% by disc diffusion.
Multidrug resistance was detected in 21.8% and 62.5% of C. jejuni and C. coli strains, respectively, by broth microdilution test and in 28.1% and 75% of C. jejuni and C. coli strains by disc diffusion method. None of the isolates showed the same pattern of multidrug resistance.
PRACTICAL APPLICATIONS
The massive use of antibiotics in modern animal husbandry for the treatment and prevention of diseases can result in the development of antibiotic resistance in zoonotic bacteria, like Campylobacter. In turn, the consumption of infected food of animal origin may represent a vehicle of transmission of resistant bacteria to humans.
The aim of this study was to ascertain the antibiotic resistance distributions of Campylobacter jejuni and Campylobacter coli strains arising from both animal and human origins, in particular resistance to antibiotics commonly used in human therapies. This can contribute to evaluate the sanitary risks of foods intended for human consumption, especially those of animal source.
- 出版日期2010-11
