BACE1 knock-outs display deficits in activity-dependent potentiation of synaptic transmission at mossy fiber to CA3 synapses in the hippocampus

作者：Wang Hui; Song Lihua; Laird Fiona; Wong Philip C; Lee Hey Kyoung^*

来源：Journal of Neuroscience, 2008, 28(35): 8677-8681.

DOI：10.1523/JNEUROSCI.2440-08.2008

• long-term potentiation
• long-term depression
• presynaptic
• paired-pulse facilitation
• beta-secretase
• Alzheimer's disease

beta-Amyloid precursor protein cleavage enzyme 1 (BACE1) has been identified as Amajor neuronal beta-secretase critical for the formation of beta-amyloid (A beta) peptide, which is thought responsible for the pathology of Alzheimer's disease (AD). Therefore, BACE1 is one of the key therapeutic targets that can prevent the progression of AD. Previous studies showed that knocking out the BACE1 gene prevents A beta formation, but results in behavioral deficits and specific synaptic dysfunctions at Schaffer collateral to CA1 synapses. However, BACE1 protein is most highly expressed at the mossy fiber projections in CA3. Here, we report that BACE1 knock-out mice display reduced presynaptic function, as measured by an increase in paired-pulse facilitation ratio. More dramatically, mossy fiber long-term potentiation (LTP), which is normally expressed viAan increase in presynaptic release, was eliminated in the knock-outs. Although long-term depression was slightly larger in the BACE1 knock-outs, it could not be reversed. The specific deficit in mossy fiber LTP was upstream of cAMP signaling and could be "rescued" by transiently elevating extracellular Ca2+ concentration. These results suggest that BACE1 may play Acritical role in regulating presynaptic function, especially activity-dependent strengthening of presynaptic release, at mossy fiber synapses.

• 出版日期2008-8-27