Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells

作者：Srdjenovic Branislava U; Slavic Marija N; Stankov Karmen M; Kladar Nebojsa V; Jovic Danica S; Seke Mariana N; Bogdanovic Visnja V

来源：Hemijska Industrija, 2015, 69(4): 425-431.

DOI：10.2298/HEMIND131218054S

<jats:p>Fullerenol (C60(OH)24) nanoparticles (FNP) have a significant role in
biomedical research due to their numerous biological activities, some of
which are cytoprotective and antioxidative properties. The aim of this study
was to measure distribution of fullerenol nanoparticles and zeta potential in
cell medium RPMI 1640 with 10% fetal bovine serum (FBS) and to investigate
the influence of FNP on Chinese hamster ovary cells (CHO-K1) survival, as
well as to determine the activity of three antioxidative enzymes:
superoxide-dismutase, glutathione-reductase and glutathione-S-transferase in
mitomycin C-treated cell line. Our investigation implies that FNP, as a
strong antioxidant, influence the cellular redox state and enzyme activities
and thus may reduce cell proliferation, which confirms that FNP could be
exploited for its use as a cytoprotective agent.[Projekat Ministarstva nauke
Republike Srbije, br. III45005 i Pokrajinski Sekretarijat za nauku i
tehnoloski razvoj Vojvodine, grant number 114-451-2056/2011-01]</jats:p>

• 出版日期2015-8