Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

作者：Lleres David; Denegri Marco; Biggiogera Marco; Ajuh Paul; Lamond Angus I^*

来源：EMBO Reports, 2010, 11(6): 445-451.

DOI：10.1038/embor.2010.64

• alternative pre-mRNA splicing
• FLIM-FRET
• CDC5L
• hnRNP-M
• spliceosome

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

• 出版日期2010-6