Size-Exclusive Nanosensor for Quantitative Analysis of Fullerene C-60

作者：Kikandi Samuel N; Okello Veronica A; Wang Qiong; Sadik Omowunmi A^*; Varner Katrina E; Burns Sarah A

来源：Environmental Science & Technology, 2011, 45(12): 5294-5300.

DOI：10.1021/es1043084

This paper presents the first development of a mass-sensitive nanosensor for the isolation and quantitative analyses of engineered fullerene (C-60) nanoparticles, while excluding mixtures of structurally similar fullerenes. Amino-modified beta-cyclodextrin (beta-CD-NH2) was synthesized and confirmed by (HNMR)-H-1 as the host molecule to isolate the desired fullerene C-60. This was subsequently assembled onto the surfaces of gold-coated quartz crystal microbalance (QCM) electrodes using N-dicyclohexylcarbodiimide/N-hydroxysuccinimide (DCC/NHS) surface immobilization chemistry to create a selective molecular configuration described as (Au)-S-(CH2)(2)-CONH-beta-CD sensor. The mass change on the sensor configuration on the QCM was monitored for selective quantitative analysis of fullerene C-60 from a C-60/C-70 mixture and soil samples. About similar to 10(14)-10(16) C-60 particles/cm(2) were successfully quantified by QCM measurements. Continuous spike of 200 mu L of 0.14 mg C-60 /mL produced changes in frequency (-Delta f) that varied exponentially with concentration. FESEM and time-of-flight secondary-ion mass spectrometry confirmed the validity of sensor surface chemistry before and after exposure to fullerene C-60. The utility of this sensor for spiked real-world soil samples has been demonstrated. Comparable sensitivity was obtained using both the soil and purified toluene samples. This work demonstrates that the sensor has potential application in complex environmental matrices.

• 出版日期2011-6-15