MicroRNA-222 promotes the proliferation and migration of cervical cancer cells

作者：Sun, Yi; Zhang, Bo; Cheng, Jiajing; Wu, Yi; Xing, Feng; Wang, Yingyi; Wang, Qingying; Qiu, Jin^*

来源：Clinical and Investigative Medicine, 2014, 37(3): E131-E141.

DOI：10.25011/cim.v37i3.21380

• TRAIL RESISTANCE
• GENE-EXPRESSION
• MIR-222
• CYCLE
• PTEN

Purpose: This study aimed to investigate the role of small non-coding RNA-222 (microRNA-222; miR-222) in the development of cervical cancer (CC). @@@ Methods: Normal and CC specimens were obtained from 18 patients. HeLa and SiHa cells were grown in Dulbecco's modified Eagle's medium. RT-PCR, Western blot, migration assay, flow cytometry and immunofluorescence microscopy were used for analyses. @@@ Results: When compared with normal cervical tissues, miR-222 was upregulated in human CC, and the extent of up-regulation was associated with the extent and depth of CC invasion. Expression of miR-222 was inversely related to the expression of phosphatase and tensin homolog (PTEN) and p27. The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells. The expression of proteins (E-cadherin and paxillin) related to the proliferation and migration was also elevated. @@@ Conclusion: MiR-222 plays an important role in the tumorigenesis of CC, possibly by specifically down-regulating p27(Kip1) and PTEN. Our findings suggest that miR-222 may serve as a new therapeutic target in CC.

• 出版日期2014
• 单位同济大学