Uremia-associated immunological aging is stably imprinted in the T-cell system and not reversed by kidney transplantation

作者：Meijers Ruud W J^*; Litjens Nicolle H R; de Wit Elly A; Langerak Anton W; Baan Carla C; Betjes Michiel G H

来源：Transplant International, 2014, 27(12): 1272-1284.

DOI：10.1111/tri.12416

• aging
• end-stage renal disease patients
• kidney transplantation
• T cells
• telomeres
• T-cell receptor excision circle

The uremia-induced inflammatory environment in end-stage renal disease (ESRD) patients is associated with premature T-cell aging resulting in a defective T-cell immunity. As kidney transplantation (KTx) reduces the pro-inflammatory environment, we hypothesized that KTx would rejuvenate the aged T-cell system. As aging parameters, we determined in 70 KTx recipients the differentiation status by immunophenotyping, thymic output by the T-cell receptor excision circle (TREC) content together with CD31(+) naive T-cell numbers and the relative telomere length (RTL) as a measure for proliferative history at pre-KTx, 3, 6 and 12months post-KTx. In addition, T-cell function was determined by measuring the proliferative capacity and percentages of cytokine-producing cells. Directly post-KTx, memory T-cell numbers were diminished but restored to pre-KTx values at 12months, except for CD4(+)EM T cells. The RTL of (memory) CD4(+) and CD8(+) T cells did not change. In contrast, TREC content and CD31(+) naive T-cell numbers were stable post-KTx although the RTL of naive CD4(+) and CD8(+) T cells decreased implying homeostatic proliferation of naive cells, in response to a temporary decrease in memory cells. The T-cell function was not improved post-KTx. Our findings demonstrate that the uremia-associated aged phenotype is stably imprinted in the T-cell system and not reversed by KTx.

• 出版日期2014-12