Anthocyanins isolated from the purple-fleshed sweet potato attenuate the proliferation of hepatic stellate cells by blocking the PDGF receptor

作者：Choi, Jae Ho; Hwang, Yong Pil; Park, Bong Hwan; Choi, Chul Yung; Chung, Young Chul^*; Jeong, Hye Gwang

来源：Environmental Toxicology and Pharmacology, 2011, 31(1): 212-219.

DOI：10.1016/j.etap.2010.10.011

• Purple-fleshed sweet potato
• Anthocyanin fraction
• Hepatic stellate cells
• PDGF
• Proliferation
• Alpha-SMA

During the process of liver fibrosis, hepatic stellate cells (HSCs) play a critical role in the increased formation and reduced degradation of extracellular matrix in the liver. We investigated the anti-proliferative effects of an anthocyanin fraction (AF), isolated from the purple-fleshed sweet potato, on platelet-derived growth factor (PDGF)-BB-dependent signaling pathways in HSC-T6 cells. HSC proliferation plays a pivotal role in liver fibrogenesis. The AF suppressed HSC activation, including PDGF-induced proliferation and alpha-smooth muscle actin (alpha-SMA) expression. Additionally, AF inhibited PDGF-BB-induced Akt and ERK1/2 phosphorylation. AF inhibited the phosphorylation level of PDGF receptor-beta (PDGFR-beta) following PDGF-BB stimulation, providing a mechanism for the inhibition of AF-mediated kinase. These results suggest that AF suppresses HSC proliferation by blocking PDGFR-beta signaling, inhibiting Akt and ERK1/2 activation and alpha-SMA expression.

• 出版日期2011-1