Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

作者：Tanaka Torahiko^*; Hasegawa Yuichiro; Saito Makoto; Ikeda Masanori; Kato Nobuyuki

来源：Journal of Bioscience and Bioengineering, 2010, 110(3): 374-376.

DOI：10.1016/j.jbiosc.2010.03.014

• Single-chain Fv
• Detergent
• Critical micelle concentration
• Hepatitis C virus
• NS4B

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

• 出版日期2010-9