An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

作者：Wen, Zhi-Bin; Liang, Wen-Bin; Zhuo, Ying; Xiong, Cheng-Yi; Zheng, Ying-Ning; Yuan, Ruo^*; Chai, Ya-Qin^*

来源：Chemical communications, 2018, 54(77): 10897-10900.

DOI：10.1039/c8cc05525d

• STRAND-DISPLACEMENT
• LIVING CELLS
• TUMOR-GROWTH
• DNA
• TOEHOLD
• APTAMER
• WORLD

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21. More importantly, the target microRNA-21 could be regenerated with ATP as the fuel rather than a nucleotide segment in conventional approaches, which made the proposed strategy simple and efficient due to the high affinity and strength of the aptamer-target interaction.

• 出版日期2018-10-4
• 单位西南大学