Association Between the Expression of Aggrecan and the Distribution of Aggrecan Gene Variable Number of Tandem Repeats With Symptomatic Lumbar Disc Herniation in Chinese Han of Northern China

作者：Cong, Lin; Pang, Hao; Xuan, Dwight; Tu, Guan Jun^*

来源：Spine, 2010, 35(14): 1371-1376.

DOI：10.1097/BRS.0b013e3181c4e022

• symptomatic lumbar disc herniation
• aggrecan gene
• proteoglycans
• aggrecan

Study Design. Case-control study.
Objective. To examine the association between the expression of aggrecan and the aggrecan gene variable number of tandem repeats (VNTR) polymorphism with symptomatic lumbar disc herniation (LDH) in Chinese Han of Northern China.
Summary of Background Data. Aggrecan fragments have been found in human degenerated discs, and an association between the aggrecan VNTR polymorphism and intervertebral disc degeneration has been previously reported in middle-aged Finnish men. However, the relationship between the munity of symptomatic LDH with the expression of aggrecan and aggrecan gene VNTR has not been well studied.
Methods. The disease group consisted of 70 patients already diagnosed with symptomatic LDH. The control group consisted of 14 patients restricted to spinal trauma and 113 healthy blood donors without symptoms of LDH who were not diagnosed with LDH. Disc tissue samples were obtained from surgical operations, and blood samples were donated from all participants. The aggrecan expression in isolated tissues was assessed by Western blot using specific antibodies. The aggrecan gene VNTR region was analyzed by polymerase chain reaction.
Results. The aggrecan expression positive rate of control group was statistically and significantly higher (P < 0.001) than that of the disease group. Moreover, there was a statistically significant higher frequency of allele 25 or allele 21 in disease group compared with controls (P-A25 = 0.003416 and P-A21 = 0.000716). Compared with the participants with 2 alleles >25 repeats, subjects with 1 or 2 alleles <= 25 repeats statistically and significantly over-represented the disease group without the expression of aggrecan (P < 0.001).
Conclusion. The findings suggest a relation between aggrecan and symptomatic LDH, where symptomatic LDH has a lower tendency of allele repeats. In addition, this study observed an association between the distribution of aggrecan gene VNTR polymorphism and the expression of aggrecan in symptomatic LDH.

• 出版日期2010-6-15
• 单位中国医科大学