A rapid LC-MS/MS method for the determination of moniliformin and occurrence of this mycotoxin in maize products from the Bavarian market

作者：Barthel Joerg^*; Rapp Martin; Holtmannspoetter Heinrich; Gottschalk Christoph

来源：Mycotoxin Research, 2018, 34(1): 9-13.

DOI：10.1007/s12550-017-0293-y

• Maize
• Mycotoxins
• Moniliformin
• LC-MS/MS HILIC

A simple LC-MS/MS method was developed for the determination of moniliformin (MON) in maize and applied for the analysis of samples within the official food surveillance. The homogenized samples were extracted with acetonitrile/water 50/50 (v/v) which proved to have the highest extraction efficiency compared to other tested solvents. The centrifuged extracts were diluted with acetonitrile and were measured after chromatographic separation by HILIC (hydrophobic interaction liquid chromatography)-HPLC without any cleanup (dilute and shoot approach). The LOD and LOQ achieved by this procedure were 2.6 and 8.8 mu g/kg, respectively. Thirty-nine samples of popcorn, maize meal, and semolina were collected in 2014 and 2015 at mills, cinemas, wholesale, and retail from the Bavarian market (Germany). The rate of contamination with MON was very high (97%) with levels ranging between the LOD and 847 mu g/kg. The mean level was 118 mu g/kg and the median, 39 mu g/kg. The maximum value was detected in maize meal. The results are discussed with respect to possible health implications for the consumer.

• 出版日期2018-3