ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF PROTEIN HYDROLYSATES FROM MYTILUS EDULIS AND ULTRAFILTRATION MEMBRANE FRACTIONS

作者：Park Soo Yeon; Ahn Chang Bum; Je Jae Young^*

来源：Journal of Food Biochemistry, 2014, 38(5): 460-468.

DOI：10.1111/jfbc.12070

Multifunctional bioactive peptides from Mytilus edulis were produced by gastrointestinal digestion. The optimal conditions for production were determined at an enzyme/substrate ratio of 1:500 for 30min hydrolysis time based on antioxidant and anti-inflammatory assays. Thus, peptides at 1:500 were further fractionated based on molecular weight. The %26lt;1kDa peptide fraction exhibited 0.25g/mL of IC50 value with the highest activity against 2,2-diphenyl-1-picrylhydrazyl scavenging activity, but similar reducing power and 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS(+)) radical scavenging activity were observed in all fractions. In the oxygen radical absorbance capacity assay, the 1-5kDa peptide fraction exhibited the best oxygen radical absorbance capacity value by 430.8M trolox equivalent/mg of sample. In addition, the 1-5kDa peptide fraction exhibited 58.74% of protection ability against hydroxyl radical-induced DNA damage. In the NO inhibition assay, the %26gt;5kDa peptide fraction showed 92.35% inhibition activity. All peptide fractions are rich in antioxidant amino acids such as Glu, Asp and His.

• 出版日期2014-10