A tandem cell for nanopore-based DNA sequencing with exonuclease

作者：Sampath G^*

来源：RSC Advances, 2015, 5(1): 167-171.

DOI：10.1039/c4ra10326b

A tandem electrolytic cell with the structure [cis1, upstream nanopore (UNP), trans1 = cis2, downstream nanopore (DNP), trans2], an exonuclease enzyme attached to the downstream side of UNP, and a chemical adapter in or a profiled voltage over DNP can be used with bandwidths of a few kHz to sequence bases in ssDNA in natural order with high accuracy and without loss in trans1/cis2 or regression into DNP from trans2.

• 出版日期2015