A novel application of the buccal micronucleus cytome assay in oral lichen planus: A pilot study

作者：Sanchez Siles M^*; Ros Llor I; Camacho Alonso F; Lopez Jornet P

来源：Archives of Oral Biology, 2011, 56(10): 1148-1153.

DOI：10.1016/j.archoralbio.2011.02.019

• Oral lichen planus
• Oral cancer
• Buccal micronucleus cytome assay

Background: The most important complication of oral lichen planus is malignancy transformation.
Objective: The aim of this study was to assess cellular and nuclear morphology in a group of patients with oral lichen planus measured by means of buccal micronucleus cytome assay.
Study design: This study included thirty patients with a clinicopathological diagnosis of oral lichen planus (all with atrophic-erosive clinical forms of OLP) and thirty healthy control subjects. Both samples were similar in age and gender. The buccal micronucleus cytome assay protocol consisted of: cell collection from both cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining followed by fluorescent microscope analysis. 2 x 10(6) exfoliated cells were screened for nuclear abnormalities: micronuclei, nuclear buds, binucleation, basal and differentiated cells, condensed chromatin, karyorrhectic cells, pyknosis and karyolytic cells.
Results: Patients with oral lichen planus showed significantly higher frequencies of micronuclei (p < 0.001), nuclear buds (p < 0.001), binucleated cells (p < 0.021) than the control group.
Conclusions: This method is an easy way for clinicians to assess DNA damage, proliferative potential of basal cells and cell death in buccal cells in cases of oral lichen planus.

• 出版日期2011-10