An efficient regeneration system for peanut: Somatic embryogenesis from embryonic leaflets

作者：Zhao Mingxia; Sui Jiongming; Qiao Lixian; Tan Lingling; Wang Jingshan^*

来源：Journal of Food Agriculture and Environment, 2012, 10(2): 527-531.

• Peanut (Arachis hypogaea L.)
• embryonic leaflet
• somatic embryogenesis
• regeneration

An efficient method of plant regeneration via somatic embryogenesis was developed for five peanut cultivars. Somatic embryogenesis was induced from embryonic leaflets on MSB5 medium (Murashige and Skoog salts plus B-5 vitamins) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). After 3 weeks, the frequency of somatic embryo formation was highest with 5 mg L-1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L-1 2,4-D for cultivar Huayu 20 (similar to 79.5%), or 10 mg L-1 42,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively). When explants with somatic embryos were subcultured on a germination medium, conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6-93.4% conversion) and next highest with thidiazuron (35.6-78.2%, depending on concentration) but was substantially lower with gibberellic acid (0-24.6%, depending on concentration), abscisic acid (0-3.9%, depending on concentration), or the control (without growth regulators; 3.2-23.8%). When 33 regenerated Huayu 23 plantlets were cleft grafted onto germinated seedlings and transplanted into the field, 31 grew to maturity and produced viable seeds.

• 出版日期2012-4
• 单位青岛农业大学