Delayed fluorescence imaging of photosynthesis inhibitor and heavy metal induced stress in potato

作者：Razinger Jaka^*; Drinovec Luka; Berden Zrimec Maja

来源：Central European Journal of Biology, 2012, 7(3): 531-541.

DOI：10.2478/s11535-012-0038-z

• Cadmium
• Chlorophyll fluorescence
• Copper
• Diuron
• EMCCD camera
• Heavy metals
• Herbicides
• Paraquat
• Photosynthesis inhibitors
• Solanum tuberosum
• Visualization

Early chemical-induced stress in Solanum tuberosum leaves was visualized using delayed fluorescence (DF) imaging. The ability to detect spatially heterogeneous responses of plant leaves exposed to several toxicants using delayed fluorescence was compared to prompt fluorescence (PF) imaging and the standard maximum fluorescence yield of PSII measurements (Fv/Fm). The toxicants used in the study were two photosynthesis inhibitors (herbicides), 100 A mu M methyl viologen (MV) and 140 A mu M diuron (DCMU), and two heavy metals, 100 A mu M cadmium and 100 A mu M copper. The exposure times were 5 and 72 h. Significant photosynthesis-inhibitor effects were already visualized after 5 h. In addition, a significant reduction in the DF/PF index was measured in DCMU- and MV-treated leaves after 5 h. In contrast, only DCMU-treated leaves exhibited a significant decrease in Fv/Fm after 5 h. All treatments resulted in a significant decrease in the DF/PF parameter after 72 h of exposure, when only MV and Cd treatment resulted in visible symptoms. Our study highlights the power of delayed fluorescence imaging. Abundant quantifiable spatial information was obtained with the instrumental setup. Delayed fluorescence imaging has been confirmed as a very responsive and useful technique for detecting stress induced by photosynthesis inhibitors or heavy metals.

• 出版日期2012-6